The CD103-XCR1 axis mediates the recruitment of immunoregulatory dendritic cells after traumatic injury

Lokwani, R.; Ngo, T. B.; DeStefano, S.; Adusei, K. M.; Bhuiyan, M.; Josyula, A.; Faust, M.; Lin, A.; Karkanitsa, M.; Fathi, P.; Sadtler, K.

doi:10.1101/2022.08.19.504399

bioRxiv · 10.1101/2022.08.19.504399

The CD103-XCR1 axis mediates the recruitment of immunoregulatory dendritic cells after traumatic injury

Lokwani, R.·Ngo, T. B.·DeStefano, S.·Adusei, K. M.·Bhuiyan, M.·Josyula, A.·Faust, M.·Lin, A.·Karkanitsa, M.·Fathi, P.·Sadtler, K.

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Abstract

During wounding and material implantation there is a disturbance in tissue homeostasis and release of self-antigen, and regulation between tolerance and auto-inflammation in injury is not well understood. Here, we analyzed antigen-presenting cells in biomaterial-treated muscle injury and found that pro-regenerative materials enrich Batf3-dependent CD103+XCR1+CD301b+ dendritic cells associated with cross-presentation and self-tolerance. Muscle trauma was accompanied by CD8+ iTregs and expansion of CD103+XCR1+CD62L- adaptive immune cells. Up-regulation of E-Cadherin (the ligand for CD103) and XCL-1 in injured tissue suggests a mechanism for cell recruitment to trauma. Without cross-presenting cells T cell activation increases, pro-regenerative macrophage polarization decreases, and muscle healing is impaired. These data describe a regulatory communication network through CD103+XCR1+ immune cells resulting in downstream effects on tissue regeneration.

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Lokwani, R., Ngo, T. B., DeStefano, S., Adusei, K. M., Bhuiyan, M., Josyula, A., Faust, M., Lin, A., Karkanitsa, M., Fathi, P., Sadtler, K.. 2022-08-19. The CD103-XCR1 axis mediates the recruitment of immunoregulatory dendritic cells after traumatic injury. https://doi.org/10.1101/2022.08.19.504399

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