Sirt3 enhanced Bufalin sensitivity of colorectal cancer cells through P53-dependent pathway of apoptosis Sirt3 enhanced Bufalin sensitivity of colorectal cancer cells through P53-dependent pathway of apoptosis

Li, L.; Liu, C.; Zhang, X.; Meng, X.; Geng, Y.; Shi, Z.; Zhang, Y.

doi:10.1101/2022.08.16.504176

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bioRxiv · 10.1101/2022.08.16.504176

Sirt3 enhanced Bufalin sensitivity of colorectal cancer cells through P53-dependent pathway of apoptosis Sirt3 enhanced Bufalin sensitivity of colorectal cancer cells through P53-dependent pathway of apoptosis

Li, L.·Liu, C.·Zhang, X.·Meng, X.·Geng, Y.·Shi, Z.·Zhang, Y.

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Abstract

Sirt3, one of class III histone deacetylase, is mainly localized in mitochondria and plays a significant role in the control of the metabolic activity, senescence and death [1]. Recently, Sirt3 emerged as a novel member of anticancer. However, the role of Sirt3 in colorectal cancer (CRC) has never been explained exactly. In this study, we found that sirt3 is down-regulated after Bufalin treatment. We also found that AC-P53 is up-regulated which induces Bax translocation to mitochondrion and open the mitochondrial permeability transition (mPTP) pores result in the release of cytochrome C lead to the activation of caspase-dependent apoptosis pathway. Collectively, our data suggests that Sirt3 may play an important role in CRC development and progression and may be a promising therapeutic target for CRC.

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Li, L., Liu, C., Zhang, X., Meng, X., Geng, Y., Shi, Z., Zhang, Y.. 2022-08-18. Sirt3 enhanced Bufalin sensitivity of colorectal cancer cells through P53-dependent pathway of apoptosis Sirt3 enhanced Bufalin sensitivity of colorectal cancer cells through P53-dependent pathway of apoptosis. https://doi.org/10.1101/2022.08.16.504176

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