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bioRxiv · 10.1101/2022.08.10.503454

Macrophage migrates on alternate non-adhesive surfaces

Xing, F.·Dong, H.·Yang, J.·Fan, C.·Hou, M.·Zhang, P.·Hu, F.·Zhou, J.·Chen, L.·Pan, L.·Xu, J.
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Abstract

Macrophages migrate across tissues upon immune demand, but their motility on heterogeneous substrates remains unclear. Protein-repelling reagents, e.g., poly(ethylene) glycol (PEG), are routinely employed to resist cell adhering and migrating. Contrary to this perception, we discovered a unique locomotion of macrophages in vitro that they overcome non-adhesive PEG gaps to reach adhesive regions in a mesenchymal mode. Adhesion to adhesive regions was a prerequisite for macrophages to perform further locomotion on the PEG regions, or else they kept a suspended round shape. Podosomes were found highly enriched on the PEG region, which supported macrophage migration. Myosin IIA played a negative role in macrophage motility. Moreover, a developed cellular Potts model reproduced the experimental observations. These findings uncovered a new migratory behavior on non-adhesive surfaces in macrophages. One-Sentence SummaryMacrophages can migrate across non-adhesive surfaces that are absolute boundaries for other cell types.

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Xing, F., Dong, H., Yang, J., Fan, C., Hou, M., Zhang, P., Hu, F., Zhou, J., Chen, L., Pan, L., Xu, J.. 2022-08-11. Macrophage migrates on alternate non-adhesive surfaces. https://doi.org/10.1101/2022.08.10.503454

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