Regioselective control of biocatalytic C-H activation and halogenation

Kissman, E. N.; Neugebauer, M. E.; Sumida, K. H.; Swenson, C. V.; Sambold, N. A.; Marchand, J. A.; Millar, D. C.; Chang, M. C. Y.

doi:10.1101/2022.08.04.502814

bioRxiv · 10.1101/2022.08.04.502814

Regioselective control of biocatalytic C-H activation and halogenation

Kissman, E. N.·Neugebauer, M. E.·Sumida, K. H.·Swenson, C. V.·Sambold, N. A.·Marchand, J. A.·Millar, D. C.·Chang, M. C. Y.

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Abstract

Biocatalytic C-H activation has the potential to merge enzymatic and synthetic strategies for bond formation. FeII/KG-dependent halogenases are particularly distinguished for their ability both to control selective C-H activation as well as to direct group transfer of a bound anion along a reaction axis separate from oxygen rebound, enabling the development of new transformations. In this context, we elucidate the basis for selectivity of enzymes that perform selective halogenation to yield 4-Cl-lysine (BesD), 5-Cl-lysine (HalB), and 4-Cl-ornithine (HalD), allowing us to probe how regioselectivity and chain length selectivity are achieved. We now report the crystal structure of the HalB and HalD, revealing the key role of the substrate-lid in positioning the substrate for C4 vs C5 chlorination and recognition of lysine vs ornithine. Targeted engineering of the substrate-binding lid further demonstrates that these selectivities can be altered or switched, showcasing the potential to develop halogenases for biocatalytic applications.

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Kissman, E. N., Neugebauer, M. E., Sumida, K. H., Swenson, C. V., Sambold, N. A., Marchand, J. A., Millar, D. C., Chang, M. C. Y.. 2022-08-05. Regioselective control of biocatalytic C-H activation and halogenation. https://doi.org/10.1101/2022.08.04.502814

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