Selective Androgen Receptor Modulator Microparticle Formulation Reverses Muscle Hyperalgesia in Mouse Model of Widespread Muscle Pain

Lesnak, J. B.; Nakhla, D. S.; Plumb, A. N.; McMillan, A.; Saha, S.; Gupta, N.; Xu, Y.; Phruttiwanichakun, P.; Rasmussen, L.; Meyerholz, D. K.; Salem, A. K.; Sluka, K. A.

doi:10.1101/2022.08.02.502353

bioRxiv · 10.1101/2022.08.02.502353

Selective Androgen Receptor Modulator Microparticle Formulation Reverses Muscle Hyperalgesia in Mouse Model of Widespread Muscle Pain

Lesnak, J. B.·Nakhla, D. S.·Plumb, A. N.·McMillan, A.·Saha, S.·Gupta, N.·Xu, Y.·Phruttiwanichakun, P.·Rasmussen, L.·Meyerholz, D. K.·Salem, A. K.·Sluka, K. A.

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Abstract

Currently, there is a need for the generation of non-opioid analgesics for treating chronic pain. Preclinical and clinical studies demonstrate the analgesic effects of testosterone. However, treatment with testosterone is not feasible due to adverse effects. Selective androgen receptor modulators (SARMs) were developed to overcome these limitations by minimizing activation of androgenic side effects. First, we demonstrate SARM administration alleviates widespread muscle pain in male and female mice. We then developed a SARM-loaded PLGA microparticle formulation that reverses widespread muscle pain in two injections. In vitro and in vivo release kinetics demonstrate the microparticle formulation had sustained SARM release for 4 weeks. Antagonism of androgen receptors blocked the analgesic effects of the SARM microparticles. SARM treatment had no effect on cardiac or liver enzymes, cardiac histology, and did not produce rewarding behavior. These studies demonstrate SARM microparticles as a potential therapeutic for chronic muscle pain. One Sentence SummaryA selective androgen receptor modulator microparticle formulation alleviates widespread muscle pain in male and female mice while being non-toxic.

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Lesnak, J. B., Nakhla, D. S., Plumb, A. N., McMillan, A., Saha, S., Gupta, N., Xu, Y., Phruttiwanichakun, P., Rasmussen, L., Meyerholz, D. K., Salem, A. K., Sluka, K. A.. 2022-08-04. Selective Androgen Receptor Modulator Microparticle Formulation Reverses Muscle Hyperalgesia in Mouse Model of Widespread Muscle Pain. https://doi.org/10.1101/2022.08.02.502353

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