Origin, Prospective Identification, and Function of Circulating Endothelial Colony Forming Cells in Mouse and Human

Lin, Y.; Banno, K.; Gil, C.-H.; Myslinski, J.; Hato, T.; Shelley, W. C.; Gao, H.; Xuei, X.; Liu, Y.; Basile, D. P.; Yoshimoto, M.; Prasain, N.; Tarnawsky, S. P.; Adams, R. H.; Naruse, K.; Yoshida, J.; Murphy, M. P.; Horie, K.; Yoder, M. C.

doi:10.1101/2022.07.31.502241

bioRxiv · 10.1101/2022.07.31.502241

Origin, Prospective Identification, and Function of Circulating Endothelial Colony Forming Cells in Mouse and Human

Lin, Y.·Banno, K.·Gil, C.-H.·Myslinski, J.·Hato, T.·Shelley, W. C.·Gao, H.·Xuei, X.·Liu, Y.·Basile, D. P.·Yoshimoto, M.·Prasain, N.·Tarnawsky, S. P.·Adams, R. H.·Naruse, K.·Yoshida, J.·Murphy, M. P.·Horie, K.·Yoder, M. C.

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Abstract

Most circulating endothelial cells are apoptotic, but rare circulating endothelial colony forming cells (C-ECFCs) with proliferative and vasculogenic activity can be cultured; the origin and naive function of these C-ECFCs remains obscure. Herein, detailed lineage tracing reveals murine C-ECFCs emerge in the early postnatal period, display high vasculogenic potential, with enriched frequency of clonal proliferative cells compared to tissue-resident ECFCs, and are not committed to or derived from the bone marrow hematopoietic system but from tissue-resident ECFCs. In human subjects, C-ECFCs are present in the CD34bright cord blood mononuclear subset, possess proliferative potential and in vivo vasculogenic function in a naive or cultured state, and display a single cell transcriptome sharing some umbilical venous endothelial cell features like, higher Protein C Receptor and extracellular matrix gene expression. This study provides an advance for the field by identifying the origin, naive function, and antigens to prospectively isolate C-ECFCs for translational studies.

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Lin, Y., Banno, K., Gil, C.-H., Myslinski, J., Hato, T., Shelley, W. C., Gao, H., Xuei, X., Liu, Y., Basile, D. P., Yoshimoto, M., Prasain, N., Tarnawsky, S. P., Adams, R. H., Naruse, K., Yoshida, J., Murphy, M. P., Horie, K., Yoder, M. C.. 2022-08-01. Origin, Prospective Identification, and Function of Circulating Endothelial Colony Forming Cells in Mouse and Human. https://doi.org/10.1101/2022.07.31.502241

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