Physiological traits and their relationships vary along an aridity gradient within and among Fijian bee species

Abstract

O_LITemperature and water availability are hypothesised to be important abiotic drivers of the evolution of metabolic rates and gas exchange patterns, respectively. Specifically, the metabolic cold adaptation hypothesis (MCA) predicts that cold environments select for faster metabolic rates to counter the thermodynamics of biochemical reactions while the hygric hypothesis predicts that dry environments select for discontinuous gas exchange to reduce water loss. C_LIO_LIAlthough these two hypotheses consider different physiological traits and how they vary along different abiotic gradients, metabolic rate drives frequency of gas exchange patterns in insects meaning these two traits are inherently linked. Despite this link, the MCA and hygric hypotheses are rarely considered together and the extent to which metabolic rates and frequency of gas exchange vary and co-vary across climatic gradients remains unclear. C_LIO_LIWe tested the MCA and hygric hypotheses within a species of endemic Fijian bee, Homalictus fijiensis, across an altitudinal gradient of 1100 m, and among four Fijian bee species, including H. fijiensis, that inhabit different altitudinal bands. In Fiji, environmental temperature is [~]5{degrees}C lower in the central highlands than in the coastal lowlands with the highlands receiving [~]100 mm of additional precipitation than the lowlands each month. C_LIO_LIWe found an MCA-like pattern within H. fijiensis and among Fijian bee species, where metabolic rate decreased with increasing temperature, but precipitation also explained variation in metabolic rate. However, we did not find support for the hygric hypothesis within H. fijiensis or among species (frequency of gas exchange was not negatively correlated with precipitation). C_LIO_LIThe relationship between metabolic rate and frequency of gas exchange was steeper for species that occupied lower elevations on average, suggesting it is possible that these two traits can evolve independently of each other despite being positively correlated. C_LI