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bioRxiv · 10.1101/2022.07.23.501234

In vivo functional genomics identifies essentiality of potassium homeostasis in medulloblastoma

Abstract

The identification of cancer maintenance genes--driver genes essential to tumor survival--is fundamental for developing effective cancer therapy. Transposon-based insertional mutagenesis screens can identify cancer driver genes broadly but not discriminate maintenance from progression or initiation drivers, which contribute to cancer phenotypes and tumorigenesis, respectively. We engineered a nested, double-jumping transposon system to first dysregulate gene expression during tumorigenesis and then restore gene expression following tumor induction, allowing for genome-wide screening of maintenance essentiality in vivo. In a mouse model of medulloblastoma, the most common pediatric malignancy, insertion and remobilization of this nested transposon uncovers potassium channel genes as recurrent maintenance drivers. In human medulloblastoma, KCNB2 is the most overexpressed potassium channel across Group 3, Group 4, and SHH subgroups, and Kcnb2 knockout in mice diminishes the replicative potential of medulloblastoma-propagating cells to mitigate tumor growth. Kcnb2 governs potassium homeostasis to regulate plasma membrane tension-gated EGFR signaling, which drives proliferative expansion of medulloblastoma-propagating cells. Thus, our novel transposon system reveals potassium homeostasis as essential to tumor maintenance through biomechanical modulation of membrane signaling.

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BibTeXRIS

Fan, J. J., Wang, X., Erickson, A. W., Skowron, P., Chen, X., Shan, G., Bahrampour, S., Xiong, Y., Dong, W., Abeysundara, N., Francisco, M. A., Pusong, R. J., Suarez, R. A., Farooq, H., Holgado, B. L., Wu, X., Daniels, C., Dupuy, A. J., Cadinanos, J., Bradley, A., Bagchi, A., Moriarity, B. S., Largaespada, D. A., Morrissy, A. S., Ramaswamy, V., Mack, S. C., Garzia, L., Dirks, P. B., Wanggou, S., Li, X., Sun, Y., Taylor, M. D., Huang, X.. 2022-07-23. In vivo functional genomics identifies essentiality of potassium homeostasis in medulloblastoma. https://doi.org/10.1101/2022.07.23.501234

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