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bioRxiv · 10.1101/2022.07.22.501113

Leveraging the Elastic Deformability of Polydimethylsiloxane Microfluidic Channels for Efficient Intracellular Delivery

Abstract

With the rapid development of microfluidic based cell therapeutics systems, the need arises for compact, modular, and microfluidics-compatible intracellular delivery platforms with small footprints and minimal operational requirements. Physical deformation of cells passing through a constriction in a microfluidic channel has been shown to create transient membrane perturbations that allow passive diffusion of materials from the outside to the interior of the cell. This mechanical approach to intracellular delivery is simple to implement and fits the criteria outlined above. However, available microfluidic platforms that operate through this mechanism are traditionally constructed from rigid channels with fixed dimensions that suffer from irreversible clogging and incompatibility with larger size distributions of cells. Here we report a flexible and elastically deformable microfluidic channel, and we leverage this elasticity to dynamically generate temporary constrictions with any given size within the channel width parameters. Additionally, clogging is prevented by increasing the size of the constriction to allow clogs to pass. By tuning the size of the constriction appropriately, we show the successful delivery of GFP-coding plasmids to the interior of three mammalian cell lines and fluorescent gold nanoparticles to HEK293 FT cells all while maintaining a high cell viability rate. This development will no doubt lead to miniaturized intracellular delivery microfluidic components that can be easily integrated into larger lab-on-a-chip systems in future cell modification devices.

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BibTeXRIS

ALHMOUD, H., Alkhaled, M., Kaynak, B. E., Hanay, M. S.. 2022-07-22. Leveraging the Elastic Deformability of Polydimethylsiloxane Microfluidic Channels for Efficient Intracellular Delivery. https://doi.org/10.1101/2022.07.22.501113

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