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bioRxiv · 10.1101/2022.07.07.499158

The inceptionist's guide to base editing - de novo PAM generation to reach initially inaccessible target-sites

Pakari, K.·Wittbrodt, J.·Thumberger, T.
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Abstract

Base editing by CRISPR crucially depends on the presence of a PAM in proper distance to the editing-site. Here we present and validate an efficient one-shot approach termed "inception", relaxing these constraints. This is achieved by sequential, combinatorial base editing: de novo generated synonymous, non-synonymous or intronic PAM-sites facilitate subsequent base editing at nucleotide positions inaccessible before, increasing the targeting range of highly precise editing approaches.

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Pakari, K., Wittbrodt, J., Thumberger, T.. 2022-07-07. The inceptionist's guide to base editing - de novo PAM generation to reach initially inaccessible target-sites. https://doi.org/10.1101/2022.07.07.499158

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