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bioRxiv · 10.1101/2022.07.05.498887

SpliceWiz: easy, optimized, and accurate alternative splicing analysis in R

Wong, A. C.·Wong, J. J.-L.·Rasko, J. E.·Schmitz, U.
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Abstract

Despite an abundance of publicly available RNA sequencing datasets, a lack of integrated user-friendly tools hinder exploration of alternative splicing. SpliceWiz is an innovative, ultra-fast graphical R application that accurately quantifies splicing events using isoform-specific alignments. It is designed to accommodate hundreds of samples typically seen in clinical datasets. Novel event filters remove low-confidence measurements from analysis, enhancing accuracy over existing methods. Group-averaged strand-specific sequencing coverage plots enable clear visualization of group differences in alternative splicing, using a new file format with demonstrable performance improvements over the current BigWig standard. ompBAM, a C++ library upon which SpliceWiz is built, automates multi-threaded alignment file processing for R package developers. SpliceWiz is a powerful platform for diverse users to explore alternative splicing in large datasets.

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Wong, A. C., Wong, J. J.-L., Rasko, J. E., Schmitz, U.. 2022-07-06. SpliceWiz: easy, optimized, and accurate alternative splicing analysis in R. https://doi.org/10.1101/2022.07.05.498887

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