Optimization and comparison of two methods for spike train estimation in an unfused tetanic contraction of low threshold motor units

Abstract

BackgroundHuman movement is generated by activating motor units (MUs), i.e., the smallest structures that can be voluntarily controlled. Recent findings have shown imaging of voluntarily activated MUs using ultrafast ultrasound based on displacement velocity images and a decomposition algorithm. Given this, estimates of trains of twitches (unfused tetanic signals) evoked by the neural discharges (spikes) of spinal motor neurons are provided. Based on these signals, a band-pass filter method (BPM) has been used to estimate its spike train. In addition, an improved spike estimation method consisting of a continuous Haar wavelet transform method (HWM) has been suggested. However, the parameters of the two methods have not been optimized, and their performance has not been compared rigorously. MethodHWM and BPM were optimized using simulations. Their performance was evaluated based on simulations and two experimental datasets with 21 unfused tetanic contractions considering their rate of agreement, spike offset, and spike offset variability with respect to the simulated or experimental spikes. ResultsA range of parameter sets that resulted in the highest possible agreement with simulated spikes was provided. Both methods highly agreed with simulated and experimental spikes, but HWM was a better spike estimation method than BPM because it had a higher agreement, less bias, and less variation (p < 0.001). ConclusionsThe optimized HWM will be an important contributor to further developing the identification and analysis of MUs using imaging, providing indirect access to the neural drive of the spinal cord to the muscle by the unfused tetanic signals.