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bioRxiv · 10.1101/2022.07.01.498410

Cytotoxic effect of Rotheca serrata on cancer cell lines MCF-7 and Neuroblastoma SH-SY5Y

Patil, S. A.·Gadade, J. P.
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Abstract

Rotheca serrata (Lamiaceae), a highly medicinal plant is used as an antidote for snakebite and the plant possesses medicinal properties like hepatoprotective, antitussive, antioxidant, anticancer, neuro-protective, used in rheumatoid arthritis and is also a -glucoside inhibitor. This work aimed to study the anticancerous effect of Rotheca serrata (root and leaf) on cancer cell lines MCF-7 (breast cancer cell line) and Neuroblastoma SH-SY5Y. The results indicated that the Methanolic extract of Rotheca serrata (root and leaf) showed high anticancer activity. Different concentrations of plant extracts (25, 50, 100, 200, 400 g/ml) were used to study the anticancerous activity, amongst which the significant results were obtained for 400 g/ml concentration (both root & leaf). Effective anticancer activity against MCF - 7 breast cancer cells was shown in methanoilc extracts and were expressed as IC 50 values; in root (IC 50 value= 61.8259 {+/-} 7.428 g/ml) and in leaf (IC 50 value = 78.1497 {+/-} 6.316g/ml). The MTT assay in case of neuroblastoma (SH-SY5Y) cell lines revealed that 400g/ml concentration of leaf methanolic extract showed effective inhibition of cancer cells with IC 50 value 37.8462 {+/-} 2.957 g/ml as compared to IC 50 value of root methanolic extract which was 57.0895 {+/-} 2.351 g/ml.

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Patil, S. A., Gadade, J. P.. 2022-07-03. Cytotoxic effect of Rotheca serrata on cancer cell lines MCF-7 and Neuroblastoma SH-SY5Y. https://doi.org/10.1101/2022.07.01.498410

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