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bioRxiv · 10.1101/2022.06.30.498286

Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule symbiosis establishment

Liu, Z.·Kong, X.·Long, Y.·Zhang, H.·Jia, J.·Qiu, L.·Zhai, J.·Yan, Z.
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Abstract

Legumes form symbiosis with rhizobium leading to the development of nitrogen-fixing nodules. By integrating single-nucleus and spatial transcriptomics, we established a cell atlas of soybean nodules and roots. In central infected zone of nodule, we found that uninfected cells specialize into functionally distinct sub-groups during nodule development and revealed a transitional subtype of infected cells with enriched nodulation-related genes. Overall, our results provide a single-cell perspective for understanding rhizobium-legume symbiosis.

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Liu, Z., Kong, X., Long, Y., Zhang, H., Jia, J., Qiu, L., Zhai, J., Yan, Z.. 2022-07-02. Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule symbiosis establishment. https://doi.org/10.1101/2022.06.30.498286

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