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bioRxiv · 10.1101/2022.06.28.497868

Design of a highly specific glutamine sensor by splitting the glutamine-binding protein

Seo, M.-H.·Lim, Y.·Jung, Y. H.·Lee, J. H.·Baek, M. S.·Jung, J. H.·Kim, H.-Y.·Lee, W.·Park, K.
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Abstract

Studies on glutamine (Gln) metabolism have illuminated the vital role of Gln in cellular functions and its potential as a biomarker for disease detection. Despite the increasing interest in Gln metabolism, in-depth evaluations are challenging owing to limitations of conventional Gln-measuring methods. Thus, we developed a ligand-induced dimerization-based sensor for Gln, termed Q-SHINE, by splitting a glutamine binding protein into two separate domains. Q-SHINE enables highly accurate and convenient measurement of Gln concentration in bio-fluid samples, and the detection range is optimal for physiological Gln levels. Genetically encoded Q-SHINE sensors could also visualize intracellular Gln levels and quantify cytoplasmic and mitochondrial Gln change in living cells, which enabled detection of various cell responses to extracellular Gln supplement.

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Seo, M.-H., Lim, Y., Jung, Y. H., Lee, J. H., Baek, M. S., Jung, J. H., Kim, H.-Y., Lee, W., Park, K.. 2022-07-01. Design of a highly specific glutamine sensor by splitting the glutamine-binding protein. https://doi.org/10.1101/2022.06.28.497868

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