Convergent Gene Expression Patterns During Compatible Interactions Between Two Pseudomonas syringae Pathovars and a Common Host (Nicotiana benthamiana)

Abstract

Summary/Abstract Pseudomonas syringae is a diverse phytopathogenic species complex, and includes strains that can cause disease across a wide variety of plant species. Much previous research into the molecular basis of immunity and infection has focused on pathogen and plant responses in a handful of model strains and hosts, and with a tacit assumption that early steps in infection and host resistance are generalizable to the species complex and across plant hosts as a whole. Here, we provide a test of this assumption by measuring the dual pathogen and host transcriptomes of two distinct pathogenic lineages of P. syringae during compatible infection of a shared model host (Nicotiana benthamiana). Our results demonstrate that, with a handful of exceptions, host plants largely respond in a similar way to both pathogenic lineages and both bacterial pathogens possess highly similar transcriptional responses at 5 hours post inoculation. However, we also highlight that subsets of genes with differential expression patterns in both bacteria and host which likely represent strain-specific responses.