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bioRxiv · 10.1101/2022.06.26.496474

MenDEL: PCR Primer Design as Constrained Optimization Process

German, S.·Mitchell, L.·Vela Gartner, A.·Fenyo, D.·Boeke, J. D.
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Abstract

MotivationThe synthesis of large DNA assemblies has applications in biotechnology, and can help us better understand genome biology. These large DNA assemblies are often constructed from many smaller DNA segments, and it is critical to assess that they are correctly assembled. One low cost and rapid method to ensure that the connection between each segment is correct is to use PCR with primer pairs that span assembly junctions. However, the design of PCR primers for large assemblies consisting of multiple segments, and therefore containing multiple assembly junctions, is a challenging process. Rule-based automation of the process often results in finding primers that satisfy general criteria, but are not necessarily the best fit for every particular junction. ResultsWe have developed MenDEL - a web-based DNA design application, that provides a primer pair computation tool for multiple assembly junctions in such a way that for each junction we automatically pick the optimal pair of primers based on user specified constraints. Availability and ImplementationThe MenDEL application is available at https://mendel-isg.nyumc.org to registered users, and the code base for computing junction primers is available at https://github.com/MendelProject/PrimerOptimization.

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German, S., Mitchell, L., Vela Gartner, A., Fenyo, D., Boeke, J. D.. 2022-06-29. MenDEL: PCR Primer Design as Constrained Optimization Process. https://doi.org/10.1101/2022.06.26.496474

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