Novel strains of Campylobacter cause diarrheal outbreak in Rhesus macaques (Macaca mulatta) of Kathmandu Valley

Napit, R.; Manandhar, P.; Poudel, A.; Rajbhandari, P. G.; Watson, S.; Shakya, S.; Pradhan, S. M.; Sharma, A. N.; Johnson, C. K.; Mazet, J. K.; Karmacharya, D.

doi:10.1101/2022.06.20.496768

bioRxiv · 10.1101/2022.06.20.496768

Novel strains of Campylobacter cause diarrheal outbreak in Rhesus macaques (Macaca mulatta) of Kathmandu Valley

Napit, R.·Manandhar, P.·Poudel, A.·Rajbhandari, P. G.·Watson, S.·Shakya, S.·Pradhan, S. M.·Sharma, A. N.·Johnson, C. K.·Mazet, J. K.·Karmacharya, D.

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Abstract

Campylobacter spp. is often underreported and underrated bacteria that present real health risks to both humans and animals, including non-human primates. It is a commensal microorganism of gastrointestinal tract known to cause gastroenteritis in humans. Commonly found in many wild animals including non-human primates (monkeys-Rhesus macaques) these pathogens are known to be a common cause of diarrhea in humans in many parts of developing and under developed countries. Rhesus macaques from the two holy sites in Kathmandu (Pashupati and Swoyambhu) were included in this cross-sectional study. Opportunistic diarrheal samples of monkeys were analyzed to detect and characterize the pathogen using 16S rRNA-based PCR screening, followed by DNA sequencing and phylogenetic analysis. Out of a total 67 collected diarrheal samples, Campylobacter spp. were detected in the majority of the samples (n=64; 96%). DNA sequences of the amplified PCR products were successfully obtained from 13 samples. Phylogenetic analysis identified Candidatus Campylobacter infans (n=10, Kimura-2 parameter (K2P) pairwise distance values of 0.002287). Remaining three sequences might potentially belong to a novel Campylobacter species/sub-species-closely relating to known species of C. helviticus (K2P pairwise distance of 0.0267). Both Candidatus Campylobacter infans and C. helvitucus are known to infect humans and animals. Additionally, we also detected the bacteria in water and soil samples from the sites. Campylobacter spp. caused the 2018 diarrhea outbreak in Rhesus macaques in the Kathmandu valley. Campylobacter might be one of the important contributing pathogens in diarrheal outbreaks-both in humans and animals (monkeys) in Nepal. Due to close interactions of these animals with humans and other animals, One Health approach might be the most effective way to prevent and mitigate the threat posed by this pathogen.

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Napit, R., Manandhar, P., Poudel, A., Rajbhandari, P. G., Watson, S., Shakya, S., Pradhan, S. M., Sharma, A. N., Johnson, C. K., Mazet, J. K., Karmacharya, D.. 2022-06-20. Novel strains of Campylobacter cause diarrheal outbreak in Rhesus macaques (Macaca mulatta) of Kathmandu Valley. https://doi.org/10.1101/2022.06.20.496768

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