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bioRxiv · 10.1101/2022.06.20.496565

Pregabalin silences oxaliplatin-activated sensory neurons to relieve cold allodynia

Iseppon, F.·Luiz, A. P.·Linley, J. E.·Wood, J. N.
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Abstract

Oxaliplatin is a platinum-based chemotherapeutic agent that causes cold and mechanical allodynia in up to 90% of patients. Silent NaV1.8-positive nociceptive cold sensors have been shown to be unmasked by oxaliplatin and other neuropathic insults. This event has been causally linked to the development of cold and mechanical allodynia. Pregabalin is an anti-epileptic and analgesic drug that acts through a calcium channel 2{delta}-1 subunit to lower neurotransmitter release. Recent data also suggest pregabalin can act on NMDA receptors and other proteins, but the site of analgesic action has been considered to be the central nervous system. We examined the effects of pregabalin on oxaliplatin-evoked unmasking of cold sensitive neurons using mice expressing GCaMP-3 driven by a Pirt promoter in all sensory neurons. We found that in mice treated with oxaliplatin, intravenous injection of pregabalin significantly decreased cold allodynia. Interestingly, pregabalin also decreased the number of sensory neurons responding to cold nociceptive stimuli by altering their excitability and their temperature thresholds. These silenced neurons are medium/large cells responding to both painful mechanical and cold stimuli, corresponding to the "silent" cold sensors that become active in numerous neuropathic pain models. Deletion of 2{delta}-1 subunits abolished the effects of pregabalin on both cold allodynia and the silencing of sensory neuron unmasked by oxaliplatin. Taken together, these results define a novel, peripheral inhibitory effect of pregabalin on the excitability of silent cold-sensing neurons in a model of oxaliplatin-dependent cold allodynia. Abbreviated SummaryIseppon et al. report a novel, peripheral effect of pregabalin on oxaliplatin-dependent cold allodynia. The drug exerts its effect by silencing a specific sub-population of neurons responding to cold and mechanical stimuli in the dorsal root ganglion, and this effect is dependent on the 2{delta}-1 subunit of voltage-gated calcium channels.

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Iseppon, F., Luiz, A. P., Linley, J. E., Wood, J. N.. 2022-06-20. Pregabalin silences oxaliplatin-activated sensory neurons to relieve cold allodynia. https://doi.org/10.1101/2022.06.20.496565

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