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bioRxiv · 10.1101/2022.06.17.496643

Preclinical safety evaluation of continuous UV-A lighting in an operative setting

Guenter, R.·Zheng-Pywell, R.·Herring, B.·Murphy, M. S.·Benner, K.·Rose, J. B.
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Abstract

BackgroundGermicidal ultraviolet (UV-C) light has been shown as an effective modality for disinfection in laboratory settings and in the operative room. Traditionally, short-wavelength UV-C devices, which have previously been shown to cause DNA damage, are utilized only for disinfection in pre- and post-operative settings and are not continuously active during operations. Continuous use of intraoperative UV light can potentially decrease pathogens and subsequent surgical site infections (SSIs), which arise in approximately 5-15% of operative cases. SSIs are a significant determinant of patient morbidity, readmission rates, and overall cost. Therefore, a method of UV light disinfection with a low risk of DNA damage is needed so that greater antimicrobial protection can be afforded to patients during the entirety of their surgical procedures. A new disinfection device that harnesses longer-wavelength UV-A light to disinfect the surgical field throughout the entirety of the procedure, including pre- and post-operation, has been developed. MethodsThis study aimed to determine if intraoperatively administered UV-A light was safe, as defined by the minimal presence of DNA damage and safe amounts of reflection upon medical personnel. Using in vitro models, we examined the differential impacts of UV-C and UV-A light on DNA damage and repair pathways. In a murine model, we looked at the difference in production of DNA damage photoproducts between UV-A and UV-C exposure. ResultsOur results show UV-A light does not induce a significant amount of DNA damage at the cellular or tissue level. Furthermore, a preclinical porcine study showed that surgical personnel were exposed to safe levels of UV-A irradiance from an overhead UV-A light used during an operation. The amount of UV-A transmitted through surgical personal protective equipment (PPE) also remained within safe levels. ConclusionsIn conclusion, we found that UV-A may be a safe for intraoperative use.

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Guenter, R., Zheng-Pywell, R., Herring, B., Murphy, M. S., Benner, K., Rose, J. B.. 2022-06-18. Preclinical safety evaluation of continuous UV-A lighting in an operative setting. https://doi.org/10.1101/2022.06.17.496643

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