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bioRxiv · 10.1101/2022.06.17.496590

Structural basis for RNA-mediated assembly of type V CRISPR-associated transposons

Schmitz, M.·Querques, I.·Oberli, S.·Chanez, C.·Jinek, M.
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Abstract

CRISPR systems have been co-opted by Tn7-like elements to direct RNA-guided transposition. Type V-K CRISPR-associated transposons rely on the concerted activities of the pseudonuclease Cas12k, the AAA+ ATPase TnsC, the Zn-finger protein TniQ, and the transposase TnsB. Here we present a cryo-electron microscopic structure of a target DNA-bound Cas12k-transposon recruitment complex comprising RNA-guided Cas12k, TniQ, TnsC and, unexpectedly, the ribosomal protein S15. Complex assembly on target DNA results in complete R-loop formation mediated by critical interactions between TniQ and the trans-activating crRNA, and is coupled with TniQ-dependent nucleation of a TnsC filament. In vivo transposition assays corroborate our structural findings, and biochemical and functional analyses of S15 supports its role as a bona fide component of the type V crRNA-guided transposition machinery. Altogether, our work uncovers key aspects of the mechanisms underpinning RNA-mediated assembly of CRISPR-associated transposons that will guide their development as programmable site-specific gene insertion tools.

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Schmitz, M., Querques, I., Oberli, S., Chanez, C., Jinek, M.. 2022-06-17. Structural basis for RNA-mediated assembly of type V CRISPR-associated transposons. https://doi.org/10.1101/2022.06.17.496590

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