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bioRxiv · 10.1101/2022.06.15.496006

A single-component luminescent biosensor for the SARS-CoV-2 spike protein

Ravalin, M.·Roh, H.·Suryawanshi, R.·Kumar, G. R.·Pak, J. E.·Ott, M.·Ting, A. Y.
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Abstract

Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of E. coli and S. cerevisiae. This biosensor is deployed in multiple homogenous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an un-augmented cell phone camera. Binding Activated Tandem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest.

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Ravalin, M., Roh, H., Suryawanshi, R., Kumar, G. R., Pak, J. E., Ott, M., Ting, A. Y.. 2022-06-15. A single-component luminescent biosensor for the SARS-CoV-2 spike protein. https://doi.org/10.1101/2022.06.15.496006

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