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bioRxiv · 10.1101/2022.06.12.495838

Melanopsin activates divergent phototransduction pathways in ipRGC subtypes

Contreras, E.·Sonoda, T.·Birnbaumer, L.·M. Schmidt, T.
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Abstract

Melanopsin signaling within ipRGC subtypes impacts a broad range of behaviors from circadian photoentrainment to conscious visual perception. Yet, how melanopsin phototransduction within M1-M6 ipRGC subtypes impacts cellular signaling to drive diverse behaviors is still largely unresolved. The identity of the phototransduction channels in each subtype is key to understanding this central question but has remained controversial. In this study, we resolve two opposing models of M4 phototransduction, demonstrating that hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are dispensable for this process and providing support for a model involving melanopsin-dependent potassium channel closure and canonical transient receptor potential (TRPC) channel opening. Surprisingly, we find that HCN channels are likewise dispensable for M2 phototransduction, contradicting the current model. We instead show that in M2 phototransduction, TRPC channels act in conjunction with T-type voltage-gated calcium channels, a novel melanopsin phototransduction target. Collectively, this work resolves key discrepancies in our understanding of ipRGC phototransduction pathways in multiple subtypes and adds to mounting evidence that ipRGC subtypes employ diverse phototransduction cascades to fine-tune cellular responses for downstream behaviors.

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Contreras, E., Sonoda, T., Birnbaumer, L., M. Schmidt, T.. 2022-06-14. Melanopsin activates divergent phototransduction pathways in ipRGC subtypes. https://doi.org/10.1101/2022.06.12.495838

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