The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation

Alshafie, W.; Fotouhi, M.; Shlaifer, I.; Edwards, A.; Durcan, T.; McPherson, P. S.; Laflamme, C.

doi:10.1101/2022.06.03.494699

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bioRxiv · 10.1101/2022.06.03.494699

The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation

Alshafie, W.·Fotouhi, M.·Shlaifer, I.·Edwards, A.·Durcan, T.·McPherson, P. S.·Laflamme, C.

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Abstract

TBK1 is a serine-threonine kinase that has been linked to a number of diseases, including amyotrophic lateral sclerosis and frontotemporal dementia. Reproducible research on TBK1 has been hampered by the lack of well characterized antibodies. In this study, we characterized 11 commercial antibodies for immunoblot, immunofluorescence and immunoprecipitation, using a knock-out cell line as the control. For each application, we identified several potent and selective antibodies that will facilitate studies on TBK1.

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Alshafie, W., Fotouhi, M., Shlaifer, I., Edwards, A., Durcan, T., McPherson, P. S., Laflamme, C.. 2022-06-03. The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation. https://doi.org/10.1101/2022.06.03.494699

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