Antiviral activity of the hemolymph of Podalia sp and M. albicolis (Lepidoptera: Megalopigydae)

Carvalho, N. D.; Curti, S. P.; Oliveira, M. I.; Rofatto, H. K.; Figueiredo, C. A.; Sena de Villar, K.; Magnelli, R. F.; Mendonca, R. Z.

doi:10.1101/2022.05.25.493399

bioRxiv · 10.1101/2022.05.25.493399

Antiviral activity of the hemolymph of Podalia sp and M. albicolis (Lepidoptera: Megalopigydae)

Carvalho, N. D.·Curti, S. P.·Oliveira, M. I.·Rofatto, H. K.·Figueiredo, C. A.·Sena de Villar, K.·Magnelli, R. F.·Mendonca, R. Z.

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Abstract

Potent antiviral activity against measles, influenza, picornavirus and herpes simplex viruses was observed in the hemolymph of Podalia sp and M. Albicolis (Lepidoptera: Megalopigydae). The antiviral proteins responsible for this activity were isolated by gel filtration chromatography using a gel filtration column system (Superdex 75) and further fractionated using a Resource-Q ion exchange column system. Experiments with the semi-purified protein led to a 128-fold reduction in picornavirus production, 64-fold reduction in measles virus production and a 32-fold reduction in influenza virus replication. qPCR showed a significantly lower level of herpes virus transcription. In addition no citotoxicity and genotoxicity effect was observed for Vero cells, suggesting a very interesting potential antiviral activity.

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Carvalho, N. D., Curti, S. P., Oliveira, M. I., Rofatto, H. K., Figueiredo, C. A., Sena de Villar, K., Magnelli, R. F., Mendonca, R. Z.. 2022-05-26. Antiviral activity of the hemolymph of Podalia sp and M. albicolis (Lepidoptera: Megalopigydae). https://doi.org/10.1101/2022.05.25.493399

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