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bioRxiv · 10.1101/2022.05.25.493368

Analysis Of Redox Status At The Erythrocyte Level

Adorbley, B.·Preko, M. O.·Fodil, F.·Kharoubi, O.
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Abstract

Erythrocytes constitute a significant target for oxidative stress because of their principal function as oxygen-carrying cells. Erythrocytes are constantly exposed to reactive oxygen species as they circulate through the bloodstream. As a result, these erythrocytes have developed a complex antioxidant defense system that comprises both enzymatic and non-enzymatic antioxidants, which mitigates and maintains the erythrocytes in a redox state. This study aimed to explore the in vitro analysis of the redox status by determining the activities of Catalase, Glutathione S transferase, Thiobarbituric Acid reactive species, and total proteins at the erythrocyte level after exposure to aluminum. The experiment was carried out by incubation of erythrocytes. The erythrocytes were distributed into sterile Petri dishes in four groups (control group, Al intoxicated group, and intoxicated with Al and treated with quercetin groups (2mg/l and 5mg/l). The experiment was observed over three intervals (24H, 48H, and 72H). Evaluations were made on oxidative stress and biochemical parameters. Results obtained showed that Al treatment significantly increased the activity of thiobarbituric acid reactive substance and decreased the activity of glutathione S-transferase (GST), activities of catalase, and protein in the erythrocyte of rats. Following administration with quercetin significantly decreased the levels of free radicals and increased the activity of GST, Catalase, and protein activities. Quercetin alleviated the toxic effects of aluminum on the studied parameters. These results indicate that Al-induced oxidative stress in the erythrocytes of rats. Following administration of quercetin, the activities of the oxidative stress parameters tested were improved significantly, indicating the overall good antioxidant properties of the quercetin. In conclusion, quercetin proved to be an effective antioxidant against free radicals produced in the erythrocytes due to aluminum exposure. HIGHLIGHTSO_LIErythrocytes are exposed to reactive Oxygen Species as they circulate in the bloodstream. C_LIO_LIAluminum induces oxidative stress in erythrocytes of Wistar rats. C_LIO_LIAluminum increases the activity of TBARS and decreases the activity of GST and CAT in incubated erythrocytes. C_LIO_LIQuercetin alleviates the toxic effects of aluminum on biochemical and oxidative stress parameters. C_LI

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Adorbley, B., Preko, M. O., Fodil, F., Kharoubi, O.. 2022-05-25. Analysis Of Redox Status At The Erythrocyte Level. https://doi.org/10.1101/2022.05.25.493368

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