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bioRxiv · 10.1101/2022.05.17.492318

The Proteomic Landscape of Genome-Wide Genetic Perturbations

Abstract

Functional genomic strategies help to address the genotype phenotype problem by annotating gene function and regulatory networks. Here, we demonstrate that combining functional genomics with proteomics uncovers general principles of protein expression, and provides new avenues to annotate protein function. We recorded precise proteomes for all non-essential gene knock-outs in Saccharomyces cerevisiae. We find that protein abundance is driven by a complex interplay of i) general biological properties, including translation rate, turnover, and copy number variations, and ii) their genetic, metabolic and physical interactions, including membership in protein complexes. We further show that combining genetic perturbation with proteomics provides complementary dimensions of functional annotation: proteomic profiling, reverse proteomic profiling, profile similarity and protein covariation analysis. Thus, our study generates a resource in which nine million protein quantities are linked to 79% of the yeast coding genome, and shows that functional proteomics reveals principles that govern protein expression. Highlights- Nine million protein quantities recorded in ~4,600 non-essential gene deletions in S. cerevisiae reveal principles of how the proteome responds to genetic perturbation - Genome-scale protein expression is determined by both functional relationships between proteins, as well as common biological responses - Broad protein expression profiles in slow-growing strains can be explained by chromosomal aneuploidies - Protein half-life and ribosome occupancy are predictable from protein abundance changes across knock-outs - Functional proteomics annotates missing gene function in four complementary dimensions

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BibTeXRIS

Messner, C. B., Demichev, V., Muenzner, J., Aulakh, S., Röhl, A., Herrera-DomInguez, L., Egger, A.-S., Kamrad, S., Lemke, O., Calvani, E., Mülleder, M., Lilley, K. S., Kustatscher, G., Ralser, M.. 2022-05-18. The Proteomic Landscape of Genome-Wide Genetic Perturbations. https://doi.org/10.1101/2022.05.17.492318

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