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bioRxiv · 10.1101/2022.05.12.491149

Myofiber reconstruction at micron scale reveals longitudinal bands in heart ventricular walls

Dileep, D.·Syed, T. A.·Sloan, T. F. W.·Perundurai, D.·Siddiqi, K.·Sirajuddin, M.
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Abstract

The coordinated contraction of myocytes drives the heart to beat and circulate blood. Due to the limited spatial resolution of whole heart imaging and the piecemeal nature of high-magnification studies, a confirmed model of myofiber geometry does not yet exist. Using microscopy and computer vision we report the first three-dimensional reconstruction of myofibers across entire mouse ventricular walls at the micron scale, representing a gain of three orders of magnitude in spatial resolution over the existing models. Our analysis reveals prominent longitudinal bands of fibers that are orthogonal to the well-known circumferential ones. Our discovery impacts present understanding of heart wall mechanics and electrical function, with fundamental implications for the study of diseases related to myofiber disorganization.

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Dileep, D., Syed, T. A., Sloan, T. F. W., Perundurai, D., Siddiqi, K., Sirajuddin, M.. 2022-05-12. Myofiber reconstruction at micron scale reveals longitudinal bands in heart ventricular walls. https://doi.org/10.1101/2022.05.12.491149

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