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bioRxiv · 10.1101/2022.05.11.491437

Extending of imaging volume in soft x-ray tomography

Ekman, A.·Chen, J.-H.·Vanslembrouck, B.·Larabell, C. A.·Le Gros, M. A.·Weinhardt, V.
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Abstract

Soft x-ray tomography offers rapid whole single cell imaging with a few tens of nanometers spatial resolution without fixation or labelling. At the moment, this technique is limited to 10 {micro}m thick specimens, such that applications of soft x-ray tomography to large human cells or multicellular specimens are not possible. We have developed a theoretical and experimental framework for soft x-ray tomography to enable extension of imaging volume to 18 {micro}m thick specimens. This approach, based on long depth of field and half-acquisition tomography, is easily applicable to existing full-rotation based microscopes. This opens applications for imaging of large human cells, which are often observed in cancer research and cell to cell interactions.

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Ekman, A., Chen, J.-H., Vanslembrouck, B., Larabell, C. A., Le Gros, M. A., Weinhardt, V.. 2022-05-11. Extending of imaging volume in soft x-ray tomography. https://doi.org/10.1101/2022.05.11.491437

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