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bioRxiv · 10.1101/2022.05.09.489277

Developmentally regulated alternate 3' end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage

Berry, C. W.·Olivares, G.·Gallicchio, L.·Ramaswami, G.·Glavic, A.·Olguin, P.·Li, J. B.·Fuller, M. T.
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Abstract

Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of the 3 cleavage site, resulting in the production of mRNA isoforms with different length 3UTRs. Although widespread, the role of APA in the biology of cells, tissues and organisms has been controversial. We identified over 500 Drosophila genes that express mRNA isoforms with a long 3UTR in proliferating spermatogonia but a short 3UTR in differentiating spermatocytes due to APA. We show that the stage-specific choice of the 3 end cleavage site can be regulated by the arrangement of a canonical polyadenylation signal (PAS) near the distal cleavage site but a variant or no recognizable PAS near the proximal cleavage site. The emergence of transcripts with shorter 3UTRs in differentiating cells correlated with changes in expression of the encoded proteins, either from off in spermatogonia to on in spermatocytes or vice versa. Polysome gradient fractionation revealed over 250 genes where the long 3UTR versus short 3UTR mRNA isoforms migrated differently, consistent with dramatic stage-specific changes in translation state. Thus, the developmentally regulated choice of an alternative site at which to make the 3end cut that terminates nascent transcripts can profoundly affect the suite of proteins expressed as cells advance through sequential steps in a differentiation lineage.

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Berry, C. W., Olivares, G., Gallicchio, L., Ramaswami, G., Glavic, A., Olguin, P., Li, J. B., Fuller, M. T.. 2022-05-10. Developmentally regulated alternate 3' end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage. https://doi.org/10.1101/2022.05.09.489277

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