TMEM106B is increased in Multiple Sclerosis plaques, and deletion causes accumulation of lipid after demyelination

Shafit-Zagardo, B.; Sidoli, S.; Goldman, J. E.; DuBois, J. C.; Corboy, J. R.; Strittmatter, S. M.; Guzik, H.; Graff, S.; Nagra, R. M.

doi:10.1101/2022.05.05.490697

bioRxiv · 10.1101/2022.05.05.490697

TMEM106B is increased in Multiple Sclerosis plaques, and deletion causes accumulation of lipid after demyelination

Shafit-Zagardo, B.·Sidoli, S.·Goldman, J. E.·DuBois, J. C.·Corboy, J. R.·Strittmatter, S. M.·Guzik, H.·Graff, S.·Nagra, R. M.

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Abstract

During inflammatory, demyelinating diseases such as multiple sclerosis (MS) axonal damage is prevalent early in the disease course. Axonal damage includes swellings, defects in transport, and failure to clear damaged intracellular proteins, all of which affect recovery and compromise the integrity of neurons and remyelination. Autophagy and the clearance of damaged cell components by the proteasome are important for the maintenance of normal cellular turnover; and the restoration of cellular homeostasis. The gradual accumulation of insoluble proteins in the brain is known to impair recovery from several neurodegenerative diseases. In this study, we used mass spectrometry to identify insoluble proteins within high-speed, mercaptoethanol/sarcosyl-insoluble pellets from purified white matter plaques isolated from the brains of individuals with MS. We determined that insoluble transmembrane protein106B (TMEM106B), expressed in neurons and glia and normally lysosomal-associated, is increased in MS plaques relative to normal-appearing white matter from individuals with Alzheimers disease and non-neurologic controls. We found that decreased TMEM106B protein in mice results in significant axonal damage and lipid droplet accumulation in the spinal cord following chronic myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis. When TMEM106Bt/t mice were treated with cuprizone to experimentally induce demyelination, a significant increase in lipid deposition was observed in the corpus callosum of TMEM106Bt/t mice post-cuprizone withdrawal. Our study shows that the brain and spinal cord from challenged TMEM106Bt/t mice accumulate OilRedO+/Perilipin2+ lipid droplets. We postulate that increased insolubility of TMEM106B in MS plaques limits debris clearance by the lysosome which over time contributes to failed remyelination and axonal defects. Abbreviated AbstractTransmembrane protein106B (TMEM106B), a lysosome-associated protein, is significantly less soluble in multiple sclerosis plaques than in white matter controls. Decreased TMEM106B produces significant axonal damage and lipid accumulation in mouse models of demyelinating diseases. TMEM106B insolubility and likely loss of function may limit lysosome transport and contribute to CNS pathology.

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Shafit-Zagardo, B., Sidoli, S., Goldman, J. E., DuBois, J. C., Corboy, J. R., Strittmatter, S. M., Guzik, H., Graff, S., Nagra, R. M.. 2022-05-05. TMEM106B is increased in Multiple Sclerosis plaques, and deletion causes accumulation of lipid after demyelination. https://doi.org/10.1101/2022.05.05.490697

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