Quantum CART (qCART), a piggyBac-based system for development and production of virus-free multiplex CAR-T cell therapy

Chen, Y.-C.; Hua, W.-K.; Hsu, J. C.; Chang, P. S.; Wen, K.-L. K.; Huang, Y.-W.; Tsai, J.-C.; Kao, Y.-H.; Wu, P.-H.; Wang, P.-N.; Chen, K.-F.; Liao, W.-T.; Wu, S. C.-Y.

doi:10.1101/2022.05.03.490469

bioRxiv · 10.1101/2022.05.03.490469

Quantum CART (qCART), a piggyBac-based system for development and production of virus-free multiplex CAR-T cell therapy

Chen, Y.-C.·Hua, W.-K.·Hsu, J. C.·Chang, P. S.·Wen, K.-L. K.·Huang, Y.-W.·Tsai, J.-C.·Kao, Y.-H.·Wu, P.-H.·Wang, P.-N.·Chen, K.-F.·Liao, W.-T.·Wu, S. C.-Y.

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Abstract

Chimeric antigen receptor T (CAR-T) cell therapy has the potential to transform cancer treatment. However, CAR-T therapy application is currently limited to certain types of relapsed/refractory B cell lymphomas. To unlock the full potential of CAR-T therapy, technologic breakthroughs will be needed in multiple areas, including optimization of autologous CAR-T development, shortening the innovation cycle, and further manufacturing advancement of next-generation CAR-T therapies. Here, we established a simple and robust virus-free multiplex Quantum CART system that seamlessly and synergistically integrates four platforms: 1. GTailor for rapid identification of lead CAR construct design, 2. Quantum Nufect for effective but gentle electroporation-based gene delivery, 3. Quantum pBac, featuring a virus-free transposon-based vector with large payload capacity and integration profile similar to retrovirus, and 4. iCellar for robust and high-quality CAR+ T memory stem cell expansion. This robust, virus-free multiplex Quantum CART system is expected to unleash the full potential of CAR-T therapy for treating diseases.

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Chen, Y.-C., Hua, W.-K., Hsu, J. C., Chang, P. S., Wen, K.-L. K., Huang, Y.-W., Tsai, J.-C., Kao, Y.-H., Wu, P.-H., Wang, P.-N., Chen, K.-F., Liao, W.-T., Wu, S. C.-Y.. 2022-05-04. Quantum CART (qCART), a piggyBac-based system for development and production of virus-free multiplex CAR-T cell therapy. https://doi.org/10.1101/2022.05.03.490469

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