CD200R1 promotes IL-17 production by ILC3s, by enhancing STAT3 activation

Linley, H.; Ogden, A.; Jaigirdar, S.; Buckingham, L.; Cox, J.; Priestley, M.; Saunders, A.

doi:10.1101/2022.04.17.488595

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bioRxiv · 10.1101/2022.04.17.488595

CD200R1 promotes IL-17 production by ILC3s, by enhancing STAT3 activation

Linley, H.·Ogden, A.·Jaigirdar, S.·Buckingham, L.·Cox, J.·Priestley, M.·Saunders, A.

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Abstract

Psoriasis is a common chronic inflammatory skin disease with no cure. It is driven by the IL-23/IL-17A axis and TH17 cells but, recently group 3 innate lymphoid cells (ILC3s) have also been implicated. However, the development, and factors regulating the activity of ILC3s remain incompletely understood. Immune regulatory pathways are particularly important at barrier sites such as the skin, gut and lung, which are exposed to environmental substances and microbes. CD200R1 is an immune regulatory cell surface receptor which inhibits proinflammatory cytokine production in myeloid cells. CD200R1 is also highly expressed on ILCs, where its function remains largely unexplored. We previously observed reduced CD200R1 signalling in psoriasis skin, suggesting that dysregulation may promote disease. Here we show that contrary to this, psoriasis models are less severe in CD200R1-deficient mice due to reduced IL-17 production. Here we uncover a key cell-intrinsic role for CD200R1 in promoting IL-23-driven IL-17A production by ILC3s, by promoting STAT3 activation. CD200R1 is expressed on ILC precursors and is particularly high on neonatal ILC3s, suggesting CD200R1 may function during ILC development. Therefore, CD200R1 is required on ILC3s, potentially during their development, to promote IL-23-stimulated STAT3 activation triggering optimal IL-17 production.

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Linley, H., Ogden, A., Jaigirdar, S., Buckingham, L., Cox, J., Priestley, M., Saunders, A.. 2022-04-17. CD200R1 promotes IL-17 production by ILC3s, by enhancing STAT3 activation. https://doi.org/10.1101/2022.04.17.488595

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