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bioRxiv · 10.1101/2022.04.16.488489

Wide-Field Multicontrast Nonlinear Microscopy for Histopathology

Uribe Casta\~no, L.·Mirsanaye, K.·Golaraei, A.·Kontenis, L.·Done, S. J.·Stambolic, V.·Akens, M.·Wilson, B.·Barzda, V.
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Abstract

A multicontrast polarimetric wide-field second harmonic generation (SHG) and multiphoton excitation fluorescence (MPF) microscope is optimized for large area imaging of hematoxylin and eosin (H&E) stained and unstained histology slides. The bleaching kinetics of MPF and SHG are examined with various laser intensities at different pulse repetition rates to determine the optimal wide-filed imaging conditions for H&E stained histology slides. Several polarimetric parameters are used to investigate the organization of extracellular matrix collagen in the histology samples.

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Uribe Casta\~no, L., Mirsanaye, K., Golaraei, A., Kontenis, L., Done, S. J., Stambolic, V., Akens, M., Wilson, B., Barzda, V.. 2022-04-17. Wide-Field Multicontrast Nonlinear Microscopy for Histopathology. https://doi.org/10.1101/2022.04.16.488489

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