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bioRxiv · 10.1101/2022.04.10.487760

Photodynamic inactivation of pathogenic bacteria on human skin by applying a potent photosensitizer in a hydrogel

Eckl, D. B.·Hoffmann, A. K.·Landgraf, N.·Kalb, L.·Baessler, P.·Wallner, S.·Eichner, A.·Huber, H.·Baeumler, W.
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Abstract

The antibiotic crisis increasingly threatens the health systems world-wide. Especially as there is an innovation gap in the development of novel antibiotics, treatment options for bacterial infections become fewer. The photodynamic inactivation (PDI) of bacteria appears to be a potent, new technology that may support the treatment of colonized or infected skin. In photodynamic inactivation, a dye - called photosensitizer - absorbs light and generates reactive singlet oxygen. This singlet oxygen is then capable of killing bacteria independent of species or strain and their antibiotic resistance profile. In order to provide a practical application for the skin surface, the photosensitizer was included in an aqueous hydrogel (photodynamically active hydrogel). The efficacy of this gel was initially tested on an inanimate surface and then on the human skin ex vivo. NBTC staining and TUNEL assays were carried out on skin biopsies to investigate potential harmful effects of the surface PDI to the underlying skin cells. The photosensitizer in the gel sufficiently produced singlet oxygen while showing only little photobleaching. On inanimate surfaces as well as on the human skin, the number of viable bacteria was reduced by over or nearly up to 4 log10 steps, equal to 99.99% reduction or even more. Furthermore, histological staining showed no harmful effects of the gel towards the tissue. The application of this hydrogel represents a valuable method in decolonizing human skin including the potential to act against superficial skin infections. The presented results are promising and should lead to further investigation in a clinical study to check the effectivity of the photodynamically active hydrogel on patients.

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Eckl, D. B., Hoffmann, A. K., Landgraf, N., Kalb, L., Baessler, P., Wallner, S., Eichner, A., Huber, H., Baeumler, W.. 2022-04-10. Photodynamic inactivation of pathogenic bacteria on human skin by applying a potent photosensitizer in a hydrogel. https://doi.org/10.1101/2022.04.10.487760

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