mRNAid, an Open-Source Platform for Therapeutic mRNA Design and Optimization Strategies

Vostrosablin, N.; Lim, S.; Gopal, P.; Brazdilova, K.; Parajuli, S.; Wei, X.; Gromek, A.; Spale, M.; Muzdalo, A.; Yeo, C.; Wardyn, J.; Mejzlik, P.; Henry, B.; Partridge, A. W.; Bitton, D. A.

doi:10.1101/2022.04.04.486952

bioRxiv · 10.1101/2022.04.04.486952

mRNAid, an Open-Source Platform for Therapeutic mRNA Design and Optimization Strategies

Vostrosablin, N.·Lim, S.·Gopal, P.·Brazdilova, K.·Parajuli, S.·Wei, X.·Gromek, A.·Spale, M.·Muzdalo, A.·Yeo, C.·Wardyn, J.·Mejzlik, P.·Henry, B.·Partridge, A. W.·Bitton, D. A.

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Abstract

Recent COVID-19 vaccines unleashed the potential of mRNA-based therapeutics. mRNA optimization is indispensable for reducing immunogenicity, ensuring stability, and maximizing protein output. We present mRNAid, an experimentally validated software for mRNA optimization and visualization that generates mRNA sequences with comparable if not superior characteristics to commercially optimized sequences. To encompass all aspects of mRNA design, we also interrogated the impact of uridine content, nucleoside analogs and UTRs on expression and immunogenicity.

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Vostrosablin, N., Lim, S., Gopal, P., Brazdilova, K., Parajuli, S., Wei, X., Gromek, A., Spale, M., Muzdalo, A., Yeo, C., Wardyn, J., Mejzlik, P., Henry, B., Partridge, A. W., Bitton, D. A.. 2022-04-05. mRNAid, an Open-Source Platform for Therapeutic mRNA Design and Optimization Strategies. https://doi.org/10.1101/2022.04.04.486952

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