Integrated single-cell (phospho-)protein and RNA detection uncovers phenotypic characteristics of human antibody secreting cells.

van Buijtenen, E.; Janssen, W.; Vink, P.; Habraken, M. J. M.; Wingens, L. J. A.; van Elsas, A.; Huck, W. T. S.; van Buggenum, J. A. G. L.; van Eenennaam, H.

doi:10.1101/2022.03.31.486501

bioRxiv · 10.1101/2022.03.31.486501

Integrated single-cell (phospho-)protein and RNA detection uncovers phenotypic characteristics of human antibody secreting cells.

van Buijtenen, E.·Janssen, W.·Vink, P.·Habraken, M. J. M.·Wingens, L. J. A.·van Elsas, A.·Huck, W. T. S.·van Buggenum, J. A. G. L.·van Eenennaam, H.

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Abstract

Antibody-secreting cells (ASCs) secrete IgM, IgA, or IgG antibodies and are key components of humoral immunity; however, little is known about unique characteristics of the Ig-classes due to limited availability of material and challenges to quantify many intracellular molecular modalities at a single-cell resolution. We combined a method to in vitro differentiate peripheral B-cells into ASCs with integrated multi-omic single-cell sequencing technologies to quantify subclass-specific hallmark surface markers, transcriptional profiles and signaling transduction pathway components. Our approach detected differential expression of plasmablast and plasma cell markers, homing receptors and IL-2, IL-6, JAK/STAT and mTOR signaling activity across Ig-subclasses. Taken together, our integrated multi-omics approach allowed high-resolution phenotypic characterization of single cells in a complex sample of in vitro differentiated human ASCs. Our strategy is expected to further our understanding of human ASCs in healthy and diseased samples and provide a valuable tool to identify novel biomarkers and potential drug targets. TeaserIntegrated single-cell analysis allows tri-modal phenotypic analysis of in-vitro generated human antibody-secreting cells.

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van Buijtenen, E., Janssen, W., Vink, P., Habraken, M. J. M., Wingens, L. J. A., van Elsas, A., Huck, W. T. S., van Buggenum, J. A. G. L., van Eenennaam, H.. 2022-04-01. Integrated single-cell (phospho-)protein and RNA detection uncovers phenotypic characteristics of human antibody secreting cells.. https://doi.org/10.1101/2022.03.31.486501

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