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bioRxiv · 10.1101/2022.03.26.485946

Modulation of RNA binding properties of the RNA helicase UPF1 by its activator UPF2

Xue, G.·Maciej, V. D.·Machado de Amorim, A.·Pak, M.·Jayachandran, U.·Chakrabarti, S.
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Abstract

The NMD helicase UPF1 is a prototype of the superfamily 1 (SF1) of RNA helicases that bind RNA with high affinity and translocate on it in an ATP-dependent manner. Previous studies showed that UPF1 has a low basal catalytic activity that is greatly enhanced upon binding of its interaction partner, UPF2. Activation of UPF1 by UPF2 entails a large conformational change that switches the helicase from an RNA-clamping mode to an RNA-unwinding mode. The ability of UPF1 to bind RNA was expected to be unaffected by this activation mechanism. Here we show, using a combination of biochemical and biophysical methods, that binding of UPF2 to UPF1 drastically reduces the affinity of UPF1 for RNA, leading to a release of the bound RNA. Although UPF2 is capable of binding RNA in vitro, our results suggest that dissociation of the UPF1-RNA complex is not a consequence of direct competition in RNA binding but rather an allosteric effect that is likely mediated by the conformational change induced by binding of the helicase to its activator. We discuss these results in light of transient interactions forged during mRNP assembly, particularly in the UPF1-dependent mRNA decay pathways.

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Xue, G., Maciej, V. D., Machado de Amorim, A., Pak, M., Jayachandran, U., Chakrabarti, S.. 2022-03-27. Modulation of RNA binding properties of the RNA helicase UPF1 by its activator UPF2. https://doi.org/10.1101/2022.03.26.485946

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