Integrated protein and transcriptome high-throughput spatial profiling

Ben Chetrit, N.; Niu, X.; Swett, A. D.; Sotelo, J.; Jiao, M. S.; Roelli, P.; Stoeckius, M.; Landau, D. A.

doi:10.1101/2022.03.15.484516

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bioRxiv · 10.1101/2022.03.15.484516

Integrated protein and transcriptome high-throughput spatial profiling

Ben Chetrit, N.·Niu, X.·Swett, A. D.·Sotelo, J.·Jiao, M. S.·Roelli, P.·Stoeckius, M.·Landau, D. A.

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Abstract

Spatial transcriptomics and proteomics have independently transformed our understanding of complex biological processes; however, integration of these modalities is currently limited. To overcome this challenge, we developed Spatial PrOtein and Transcriptome Sequencing (SPOTS) for high-throughput integration of transcriptome and protein profiling within the spatial context. Applying SPOTS to spleen and breast cancer samples revealed that spatially-resolved multi-omic integration provides a comprehensive perspective on key biological processes in health and disease.

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Ben Chetrit, N., Niu, X., Swett, A. D., Sotelo, J., Jiao, M. S., Roelli, P., Stoeckius, M., Landau, D. A.. 2022-03-18. Integrated protein and transcriptome high-throughput spatial profiling. https://doi.org/10.1101/2022.03.15.484516

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