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bioRxiv · 10.1101/2022.03.15.484308

CDKL5 deficiency slows synaptic vesicle endocytosis

Kontaxi, C.·Davenport, E. C.·Kind, P. C.·Cousin, M. A.
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Abstract

Cyclin-dependent kinase-like 5 (CDKL5) deficiency disorder (CDD) is a severe early-onset epileptic encephalopathy resulting mainly from de novo mutations in the X-linked CDKL5 gene. To determine whether loss of presynaptic CDKL5 function contributes to CDD, we examined synaptic vesicle (SV) recycling in primary hippocampal neurons generated from a Cdkl5 knockout rat model. Using a genetically-encoded reporter, we revealed that CDKL5 is selectively required for efficient SV endocytosis. We showed that CDKL5 kinase activity is both necessary and sufficient for optimal SV endocytosis, since kinase-inactive mutations failed to correct endocytosis in Cdkl5 knockout neurons, whereas the isolated CDKL5 kinase domain fully restored SV endocytosis kinetics. Finally, we demonstrated that CDKL5-mediated phosphorylation of amphiphysin 1, a putative presynaptic target, is not required for CDKL5-dependent control of SV endocytosis. Overall, our findings reveal a key presynaptic role for CDKL5 kinase activity and enhance our insight into how its dysfunction may culminate in CDD.

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Kontaxi, C., Davenport, E. C., Kind, P. C., Cousin, M. A.. 2022-03-16. CDKL5 deficiency slows synaptic vesicle endocytosis. https://doi.org/10.1101/2022.03.15.484308

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