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bioRxiv · 10.1101/2022.03.07.482710

Cell Chromatography: Biocompatible chromatographic separation and interrogation of microbial cells

Hazu, M.·Ahmed, A.·Curry, E. A.·Hornby, D. P.·Gjerde, D. T.
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Abstract

The isolation of pure, single colonies lies at the heart of experimental microbiology. However, a microbial colony typically contains around one million cells at all stages of the life cycle. Here we describe a novel, cell chromatography method that facilitates the capture, purification and interrogation of microbial cells from both single and mixed cultures. The method described relies on, but is not limited to, differences in surface charge to separate bacterial strains. The method is fully biocompatible, leading to no significant loss of cell viability. The chromatographic capture of cells, combined with selective elution methods facilitates a greater level of experimental control over the sample inputs required for downstream high throughput and high sensitivity, analytical methods. The application of the method for interrogating the antibiotic resistance of bacterial strains and for the separation of bacteria from environmental samples is illustrated.

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Hazu, M., Ahmed, A., Curry, E. A., Hornby, D. P., Gjerde, D. T.. 2022-03-07. Cell Chromatography: Biocompatible chromatographic separation and interrogation of microbial cells. https://doi.org/10.1101/2022.03.07.482710

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