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bioRxiv · 10.1101/2022.03.06.482812

Revealing cell populations catching the early stages of the human embryo development in naive pluripotent stem cells

Moya-Jodar, M.·Ullate-Agote, A.·Barlabe, P.·Rodriguez-Madoz, J. R.·Abizanda, G.·Barreda, C.·Carvajal-Vergara, X.·Vilas-Zornoza, A.·Romero, J. P.·Garate, L.·Agirre, X.·Coppiello, G.·Prosper, F.·Aranguren, X.
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Abstract

Naive human pluripotent stem cells (hPSCs) are defined as the in vitro counterpart of the human preimplantation embryos epiblast and are used as a model system to study developmental processes. In this study, we report the discovery and characterization of distinct cell populations coexisting with epiblast-like cells in 5iLAF naive human induced PSCs (hiPSCs) cultures. Noteworthily these populations closely resemble different cell types of the human embryo at early developmental stages. While epiblast-like cells represented the main cell population, interestingly we detected a cell population with gene and transposable element expression profile closely resembling the totipotent 8-Cell (8C) stage human embryo, and three cell populations analogous to trophectoderm (TE) cells at different stages of their maturation process: transition, early and mature stage. Thus, 5iLAF naive hiPSCs cultures provide an excellent opportunity to model the earliest events of human embryogenesis, from the 8C stage to the peri-implantation period. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=105 SRC="FIGDIR/small/482812v2_ufig1.gif" ALT="Figure 1"> View larger version (46K): org.highwire.dtl.DTLVardef@11b680eorg.highwire.dtl.DTLVardef@4c7e0forg.highwire.dtl.DTLVardef@1e84568org.highwire.dtl.DTLVardef@24ee65_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Moya-Jodar, M., Ullate-Agote, A., Barlabe, P., Rodriguez-Madoz, J. R., Abizanda, G., Barreda, C., Carvajal-Vergara, X., Vilas-Zornoza, A., Romero, J. P., Garate, L., Agirre, X., Coppiello, G., Prosper, F., Aranguren, X.. 2022-03-06. Revealing cell populations catching the early stages of the human embryo development in naive pluripotent stem cells. https://doi.org/10.1101/2022.03.06.482812

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