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bioRxiv · 10.1101/2022.02.27.482183

Machine learning meets classical computer vision for accurate cell identification

Abstract

High-parameter multiplex immunostaining techniques have revolutionized our ability to image healthy and diseased tissues with unprecedented depth; however, accurate cell identification and segmentation remain significant downstream challenges. Identifying individual cells with high precision is a requisite to reliably and reproducibly interpret acquired data. Here we introduce CIRCLE, a cell identification pipeline that combines classical and modern machine learning-based computer vision algorithms to address the shortcomings of current cell segmentation tools for 2D images. CIRCLE is a fully automated hybrid cell detection model, eliminating subjective investigator bias and enabling high-throughput image analysis. CIRCLE accurately distinguishes cells across diverse tissues microenvironments, resolves low-resolution structures, and can be applied to any 2D image that contains nuclei. Importantly, we quantitatively demonstrate that CIRCLE outperforms current state-of-the-art image segmentation tools using multiple accuracy measures. As high-throughput multiplex imaging grows closer toward standard practice for histology, integration of CIRCLE into analysis protocols will deliver unparalleled segmentation quality.

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BibTeXRIS

Karimi, E., Rezanejad, M., Fiset, B., Perus, L., McDowell, S. A. C., Arabzadeh, A., Beugnot, G., Siegel, P., Guiot, M.-C., Quail, D. F., Siddiqi, K., Walsh, L. A.. 2022-02-28. Machine learning meets classical computer vision for accurate cell identification. https://doi.org/10.1101/2022.02.27.482183

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