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bioRxiv · 10.1101/2022.02.24.481761

AIMP1-derived peptide secreted from hair follicle stem cells activates dermal papilla cells to promote hair growth

Kim, Y.·Lee, H.·Kim, D.·Bak, S. S.·Yoon, I.·Paus, R.·Cho, S.·Jeong, S. J.·Jeon, Y.·Park, M. C.·Oh, J. W.·Park, J. M.·Kim, S. B.·Sung, Y. K.·Kim, S.
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Abstract

Hair follicle stem cells (HFSCs) and dermal papilla cells (DPCs) are crucial in the biogenesis and maintenance of hair follicles (HFs). In this study, a fragment derived from aminoacyl-tRNA synthetase-interacting multifunctional protein1 (AIMP1) was secreted from HFSCs to activate DPCs to maintain hair follicle homeostasis. A histological analysis revealed that AIMP1 levels in hair follicles decreased with hair loss. Hair regrowth in AIMP1-induced mice was faster than that in non-induced mice. Deletion mapping revealed 41 amino acids (TN41, aa 6-46) as the active region of AIMP1. The N-terminal peptide fragment of AIMP1 generated by MMP1 was secreted from Wnt-treated HFSCs to activate DPCs via FGFR2. TN41 activated Akt and ERK, increased {beta}-catenin, and enhanced DPCs activation. TN41 also promoted hair shaft elongation in cultured human hair follicles and improved the hair-inducing activity of cultured DPC spheroids. In summation, the AIMP1 fragment secreted from HFSCs appears to stimulate active hair regrowth through activating DPCs.

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Kim, Y., Lee, H., Kim, D., Bak, S. S., Yoon, I., Paus, R., Cho, S., Jeong, S. J., Jeon, Y., Park, M. C., Oh, J. W., Park, J. M., Kim, S. B., Sung, Y. K., Kim, S.. 2022-02-24. AIMP1-derived peptide secreted from hair follicle stem cells activates dermal papilla cells to promote hair growth. https://doi.org/10.1101/2022.02.24.481761

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