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bioRxiv · 10.1101/2022.02.22.481493

Spatiotemporal growth pattern during plant nutation implies fast dynamics for cell wall mechanics and chemistry: a multiscale study in Averrhoa carambola

Riviere, M.·Peaucelle, A.·Derr, J.·Douady, S.
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Abstract

Nutation is the most striking and ubiquitous example of the rhythmic nature of plant development. Although there is a consensus that this wide oscillatory motion is driven by growth, its internal mechanisms have not been fully elucidated yet. In this work, we study the specific case of nutation in compound leaves in the archetypal Averrhoa carambola plant. We quantify the macroscopic growth kinematics with time lapse imaging, image analysis and kinematics modeling. We further characterize the mechanical and chemical properties of the cell wall with atomic force microscopy and immunolabelling. Our data first reveal that the differential growth driving nutation is localized and peaks where the average growth drops. We then show this specific spatiotemporal growth profile is compatible with local contraction events. At the cell wall level, differential growth is further colocalized with an asymmetry of the cell wall elastic modulus, and with an asymmetric distribution of homogalacturonans (HG). Our results not only back up the hypothesis of HG being involved in plant growth, but also build up on it by suggesting a dynamic nature for this process. Significance StatementNutation is an oscillatory motion displayed by many organs of growing plants. Most works on nutation focus on its relation to external stimuli attempting to explain its origins. By contrast, its internal physiological mechanisms remain to be fully explored. Here we propose an experimental and multiscale characterization of undisturbed nutation. We determine the macroscopic growth profile and show it is compatible with cell expansion but also local contractions in the tissues. At the microscopic level, we reveal that both the rigidity and composition of the cell wall are asymmetrically distributed where nutation occurs. The combination of results on both scales brings contributions to the understanding of interplay between global movement, local growth, cell wall mechanics and cell wall biochemistry.

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Riviere, M., Peaucelle, A., Derr, J., Douady, S.. 2022-02-24. Spatiotemporal growth pattern during plant nutation implies fast dynamics for cell wall mechanics and chemistry: a multiscale study in Averrhoa carambola. https://doi.org/10.1101/2022.02.22.481493

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