Evolutionary trade-offs in osmotic and ionic regulation and the expression of gill ion transporter genes in high latitude, Neotropical cold clime crabs from the 'end of the world'

Abstract

Seeking to identify consequences of evolution at low temperature, we examine hyper/hypo-osmotic and ionic regulation and gill ion transporter gene expression in two sub-Antarctic Eubrachyura from the Beagle Channel, Tierra del Fuego. Despite sharing the same osmotic niche, Acanthocyclus albatrossis tolerates a wider salinity range (2-65 {per thousand}S) than Halicarcinus planatus (5-60 {per thousand}S); respective lower and upper critical salinities are 4 and 12 {per thousand}S, and 63 and 50 {per thousand}S. Acanthocyclus albatrossis is a weak hyperosmotic regulator, while H. planatus hyper-osmoconforms; isosmotic points are 1,380 and {approx}1,340 mOsm kg-1 H2O. Both crabs hyper/hypo-regulate [Cl-] well with iso-chloride points at 452 and 316 mmol L-1 Cl-, respectively. [Na+] is hyper-regulated at all salinities. mRNA expression of gill Na+/K+-ATPase is salinity-sensitive in A. albatrossis, increasing {approx}1.9-fold at 5 {per thousand}S compared to 30 {per thousand}S, decreasing at 40 to 60 {per thousand}S. Expression in H. planatus is very low salinity-sensitive, increasing {approx}4.7-fold over 30 {per thousand}S, but decreasing at 50 {per thousand}S. V(H+)-ATPase expression decreases in A. albatrossis at low and high salinities as in H. planatus. Na+-K+-2Cl- symporter expression in A. albatrossis increases 2.6-fold at 5 {per thousand}S, but decreases at 60 {per thousand}S compared to 30 {per thousand}S. Chloride uptake may be mediated by increased Na+-K+-2Cl- expression but Cl- secretion is independent of symporter expression. These unrelated eubrachyurans exhibit similar systemic osmoregulatory characteristics and are better adapted to dilute media; however, the gene expressions underlying ion uptake and secretion show marked interspecific divergences. Cold clime crabs may have limited energy expenditure by regulating hemolymph [Cl-] alone, apportioning resources for other metabolic processes. Summary statementSub-Antarctic crabs may skimp on osmoregulatory capabilities to apportion energy for metabolic processes. They regulate chloride but not sodium or osmolality. Transporter gene expressions diverge markedly. Adaptive, differential ion regulation may characterize cold clime crabs.