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bioRxiv · 10.1101/2022.02.07.479493

In Vitro Selection of Remdesivir-Resistant SARS-CoV-2 Demonstrates High Barrier to Resistance.

Checkmahomed, L.·Du Pont, V.·Riola, N. C.·Perry, J.·Li, J.·Porter, D. P.·Boivin, G.
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Abstract

In vitro selection of remdesivir-resistant SARS-CoV-2 revealed the emergence of a V166L substitution, located outside of the polymerase active site of the nsp12 protein, after 9 passages. V166L remained the only nsp12 substitution after 17 passages at a final concentration of 10 {micro}M RDV, conferring a 2.3-fold increase in EC50. When V166L was introduced into a recombinant SARS-CoV-2 virus, a 1.5-fold increase in EC50 was observed, indicating a high in vitro barrier to RDV resistance.

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Checkmahomed, L., Du Pont, V., Riola, N. C., Perry, J., Li, J., Porter, D. P., Boivin, G.. 2022-02-12. In Vitro Selection of Remdesivir-Resistant SARS-CoV-2 Demonstrates High Barrier to Resistance.. https://doi.org/10.1101/2022.02.07.479493

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