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bioRxiv · 10.1101/2022.02.02.478917

Synthetic genetic circuits enable reprogramming of plant roots

Brophy, J. A. N.·Magallon, K. J.·Kniazev, K.·Dinneny, J. R.
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Abstract

The shape of a plants root system influences its ability to reach essential nutrients in the soil and to acquire water during drought. Progress in engineering plant roots to optimize water and nutrient acquisition has been limited by our capacity to design and build genetic programs that alter root growth in a predictable manner. Here, we construct synthetic genetic circuits to control gene expression with high spatial precision across root tissues. These circuits produce specific patterns of gene expression by combining the activity of multiple native promoters using logical operations. We then use the circuits to predictably alter root structure. This work demonstrates the ability of synthetic genetic circuits to control gene expression across tissues and offers an exciting means to reprogram plant growth.

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Brophy, J. A. N., Magallon, K. J., Kniazev, K., Dinneny, J. R.. 2022-02-03. Synthetic genetic circuits enable reprogramming of plant roots. https://doi.org/10.1101/2022.02.02.478917

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