Nematodes can survive in a suspended form of life for indefinite time

Shatilovich, A.; Gade, V. R.; Pippel, M.; Hoffmeyer, T. T.; Tchesunov, A. V.; Stevens, L.; Winkler, S.; Hughes, G. M.; Traikov, S.; Hiller, M.; Rivkina, E.; Schiffer, P. H.; Myers, E. W.; Kurzchalia, T. V.

doi:10.1101/2022.01.28.478251

bioRxiv · 10.1101/2022.01.28.478251

Nematodes can survive in a suspended form of life for indefinite time

Shatilovich, A.·Gade, V. R.·Pippel, M.·Hoffmeyer, T. T.·Tchesunov, A. V.·Stevens, L.·Winkler, S.·Hughes, G. M.·Traikov, S.·Hiller, M.·Rivkina, E.·Schiffer, P. H.·Myers, E. W.·Kurzchalia, T. V.

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Abstract

Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis1 when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways1,2,3 that enable the organism to survive for prolonged periods. Recently, nematode individuals have been reanimated from Siberian permafrost after remaining in cryptobiosis. Preliminary analysis indicates that these nematodes belong to the genera Panagrolaimus and Plectus4. Here, we present precise radiocarbon dating indicating that the Panagrolaimus individuals have remained in cryptobiosis since the late Pleistocene ([~]46,000 years). Phylogenetic inference based on our genome assembly and a detailed morphological analysis demonstrate that they belong to an undescribed species, which we named Panagrolaimus n. sp. Comparative genome analysis revealed that the molecular toolkit for cryptobiosis in Panagrolaimus n. sp. and in C. elegans is partly orthologous. We show that biochemical mechanisms employed by these two species to survive desiccation and freezing under laboratory conditions are similar. Our experimental evidence also reveals that C. elegans dauer larvae can remain viable for longer periods in suspended animation than previously reported. Altogether, our findings demonstrate that nematodes evolved mechanisms potentially allowing them to suspend life over geological time scales.

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Shatilovich, A., Gade, V. R., Pippel, M., Hoffmeyer, T. T., Tchesunov, A. V., Stevens, L., Winkler, S., Hughes, G. M., Traikov, S., Hiller, M., Rivkina, E., Schiffer, P. H., Myers, E. W., Kurzchalia, T. V.. 2022-01-28. Nematodes can survive in a suspended form of life for indefinite time. https://doi.org/10.1101/2022.01.28.478251

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