A reference single-cell regulomic and transcriptomic map of cynomolgus monkeys

Qu, J.; Yang, F.; Zhu, T.; Wang, Y.; Fang, W.; Ding, Y.; Zhao, X.; Qi, X.; Xie, Q.; Xu, Q.; Xie, Y.; Sun, Y.; Chen, D.

doi:10.1101/2022.01.22.477221

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bioRxiv · 10.1101/2022.01.22.477221

A reference single-cell regulomic and transcriptomic map of cynomolgus monkeys

Qu, J.·Yang, F.·Zhu, T.·Wang, Y.·Fang, W.·Ding, Y.·Zhao, X.·Qi, X.·Xie, Q.·Xu, Q.·Xie, Y.·Sun, Y.·Chen, D.

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Abstract

Non-human primates (NHP) are attractive laboratory animal models that accurately reflect both developmental and pathological features of humans. Here we present a compendium of cell types from the cynomolgus monkey Macaca fascicularis (denoted as Monkey Atlas) using both single-cell chromatin accessibility (scATAC-seq) and RNA sequencing (scRNA-seq) data at the organism-wide level. The integrated cell map enables in-depth dissection and comparison of molecular dynamics, cell-type composition and cellular heterogeneity across multiple tissues and organs. Using single-cell transcriptomic data, we inferred pseudotime cell trajectories and cell-cell communications to uncover key molecular signatures underlying their cellular processes. Furthermore, we identified various cell-specific cis-regulatory elements and constructed organ-specific gene regulatory networks at the single-cell level. Finally, we performed a comparative analysis of single-cell landscapes among mouse, cynomolgus monkey and human, and we showed that cynomolgus monkey has significantly higher degree of cell-type similarity to human than mouse. Taken together, our study provides a valuable resource for NHP cell biology.

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Qu, J., Yang, F., Zhu, T., Wang, Y., Fang, W., Ding, Y., Zhao, X., Qi, X., Xie, Q., Xu, Q., Xie, Y., Sun, Y., Chen, D.. 2022-01-23. A reference single-cell regulomic and transcriptomic map of cynomolgus monkeys. https://doi.org/10.1101/2022.01.22.477221

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